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Servicebio Inc diamidino 2 phenylindole dapi staining
Diamidino 2 Phenylindole Dapi Staining, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dapi+stain/2+4+6+diamidino+phenylindole/pmc13049910-67-22-29
Average 86 stars, based on 1 article reviews
diamidino 2 phenylindole dapi staining - by Bioz Stars, 2026-10
86/100 stars

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Related Articles

Staining:

Article Title: LncRNA CARMN overexpression promotes prognosis and chemosensitivity of triple negative breast cancer via acting as miR143-3p host gene and inhibiting DNA replication
Article Snippet: Enhanced chemiluminescence (ECL) was performed with Immobilon Western Chemiluminescent HRP Substrate (Millipore, Billerica, USA) and Chemidoc Touching Imaging System (Biorad, California, USA) was used for observing results. .. The cells were stained with DAPI stain and β-tubulin antibody (Servicebio, Hubei, Wuhan). .. Immunofluorenscence antibody of MCM5 (11703–1-AP, anti-rabbit 1:100) was purchased from Proteintech (Chicago, USA).

Article Title: Hirsutine attenuated oxidative stress and autophagy in diabetic kidney disease through Keap1/Nrf2 pathway
Article Snippet: .. To visualize the nuclei, DAPI stain (Servicebio, Wuhan, China) was applied for 10 min at room temperature. .. Digital images of the stained sections were then acquired using a Leica TCS SP8 confocal microscope.

Article Title: Gegen-Sangshen oral liquid and its active fractions mitigate alcoholic liver disease in mice through repairing intestinal epithelial injury and regulating gut microbiota
Article Snippet: .. The nuclei were counterstained with DAPI stain (Servicebio, Wuhan, China), and finally the antifluorescence quencher (Servicebio, Wuhan, China) was added to mount, and the staining results were viewed and recorded using an Olympus BX63 fluorescence microscope (Tokyo, Japan). ..

Article Title: Exenatide regulates Th17/Treg balance via PI3K/Akt/FoxO1 pathway in db/db mice
Article Snippet: Immuno uorescence staining 5 cm-thick para n sections of pancreatic tissues were subjected to immuno uorescence staining with antibodies against IL-17 (#GB11110, RRID: AB_2892098, Servicebio, China) or Foxp3 (#GB11093, RRID: AB_2861434, Servicebio, China) in combination with anti-insulin antibody (#GB12334, RRID: AB_2916346, Servicebio, China) according to the manufacturer’s instructions. .. Nuclei were counterstained with DAPI stain (Servicebio, China). .. Immunostained images were acquired using Ortho-Fluorescent microscope imaging system (Nikon, Japan).

Article Title: Exenatide regulates Th17/Treg balance via PI3K/Akt/FoxO1 pathway in db/db mice.
Article Snippet: 5 cm-thick paraffin sections of pancreatic tissues were subjected to immunofluorescence staining with antibodies against IL-17 (#GB11110, Servicebio, China) or Foxp3 (#GB11093, Servicebio, China) in combination with anti-insulin antibody (#GB12334, Servicebio, China) according to the manufacturer’s instructions. .. Nuclei were counterstained with DAPI stain (Servicebio, China). .. Immunostained images were acquired using Ortho-Fluorescent microscope imaging system (Nikon, Japan).

Article Title: Isolation of high-purity muscle stem cells through ice-cold treatment method for the production of cell cultured meat
Article Snippet: .. This was followed by incubation at room temperature for 1 h. The cells subsequently underwent nuclear staining for 8 min using DAPI stain (Servicebio, China) at a final concentration of 2 μg/mL. .. Finally, the samples were covered using anti-fade mounting medium (Servicebio, China) and imaged using the Olympus CKX41 fluorescence inverted microscope.

Article Title: Gegen-Sangshen oral liquid and its active fractions mitigate alcoholic liver disease in mice through repairing intestinal epithelial injury and regulating gut microbiota.
Article Snippet: .. The nuclei were counterstained with DAPI stain (Servicebio, Wuhan, China), and finally the antifluorescence quencher (Servicebio, Wuhan, China) was added to mount, and the staining results were viewed and recorded using an Olympus BX63 fluorescence microscope (Tokyo, Japan). ..

Fluorescence:

Article Title: Gegen-Sangshen oral liquid and its active fractions mitigate alcoholic liver disease in mice through repairing intestinal epithelial injury and regulating gut microbiota
Article Snippet: .. The nuclei were counterstained with DAPI stain (Servicebio, Wuhan, China), and finally the antifluorescence quencher (Servicebio, Wuhan, China) was added to mount, and the staining results were viewed and recorded using an Olympus BX63 fluorescence microscope (Tokyo, Japan). ..

Article Title: Gegen-Sangshen oral liquid and its active fractions mitigate alcoholic liver disease in mice through repairing intestinal epithelial injury and regulating gut microbiota.
Article Snippet: .. The nuclei were counterstained with DAPI stain (Servicebio, Wuhan, China), and finally the antifluorescence quencher (Servicebio, Wuhan, China) was added to mount, and the staining results were viewed and recorded using an Olympus BX63 fluorescence microscope (Tokyo, Japan). ..

Microscopy:

Article Title: Gegen-Sangshen oral liquid and its active fractions mitigate alcoholic liver disease in mice through repairing intestinal epithelial injury and regulating gut microbiota
Article Snippet: .. The nuclei were counterstained with DAPI stain (Servicebio, Wuhan, China), and finally the antifluorescence quencher (Servicebio, Wuhan, China) was added to mount, and the staining results were viewed and recorded using an Olympus BX63 fluorescence microscope (Tokyo, Japan). ..

Article Title: Gegen-Sangshen oral liquid and its active fractions mitigate alcoholic liver disease in mice through repairing intestinal epithelial injury and regulating gut microbiota.
Article Snippet: .. The nuclei were counterstained with DAPI stain (Servicebio, Wuhan, China), and finally the antifluorescence quencher (Servicebio, Wuhan, China) was added to mount, and the staining results were viewed and recorded using an Olympus BX63 fluorescence microscope (Tokyo, Japan). ..

Immunofluorescence:

Article Title: Exosomes derived from Umbilical cord mesenchymal stem cell promote hair regrowth in C57BL6 mice through upregulation of the RAS/ERK signaling pathway
Article Snippet: The color development time was controlled under the microscope: the nuclei were restained, and the tissue slices were sealed by dehydration to analyze the results under a white light microscope (Olympus, IX73). .. Immunofluorescence was performed by incubating the tissue with HRP-labeled goat anti-mouse IgG secondary antibody (GB23301, Servicebio) for 50 min at room temperature, followed by the addition of DAPI stain (G1012, Servicebio) to restore the nuclei, then incubated for 10 min at room temperature. ..

Incubation:

Article Title: Exosomes derived from Umbilical cord mesenchymal stem cell promote hair regrowth in C57BL6 mice through upregulation of the RAS/ERK signaling pathway
Article Snippet: The color development time was controlled under the microscope: the nuclei were restained, and the tissue slices were sealed by dehydration to analyze the results under a white light microscope (Olympus, IX73). .. Immunofluorescence was performed by incubating the tissue with HRP-labeled goat anti-mouse IgG secondary antibody (GB23301, Servicebio) for 50 min at room temperature, followed by the addition of DAPI stain (G1012, Servicebio) to restore the nuclei, then incubated for 10 min at room temperature. ..

Article Title: Isolation of high-purity muscle stem cells through ice-cold treatment method for the production of cell cultured meat
Article Snippet: .. This was followed by incubation at room temperature for 1 h. The cells subsequently underwent nuclear staining for 8 min using DAPI stain (Servicebio, China) at a final concentration of 2 μg/mL. .. Finally, the samples were covered using anti-fade mounting medium (Servicebio, China) and imaged using the Olympus CKX41 fluorescence inverted microscope.

Concentration Assay:

Article Title: Isolation of high-purity muscle stem cells through ice-cold treatment method for the production of cell cultured meat
Article Snippet: .. This was followed by incubation at room temperature for 1 h. The cells subsequently underwent nuclear staining for 8 min using DAPI stain (Servicebio, China) at a final concentration of 2 μg/mL. .. Finally, the samples were covered using anti-fade mounting medium (Servicebio, China) and imaged using the Olympus CKX41 fluorescence inverted microscope.



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Image Search Results


Live/dead staining results in Experiment 1 (A, C, E, G) and Experiment 2 (B, D, F, H) of MG-63 cells cultured on (A, B) Glass, (C, D) Sc _plate, (E, F) Sc _v_static, (G, H) Sc _v_RPM. Living cells stained green by calcein-AM, dead cells stained red by propidium iodide. Phalloidin/DAPI staining results in Experiment 2: (B2) Glass, (D2) Sc _plate, (F2) Sc _v_static, (H2) Sc _v_RPM. Actin filaments stained green by phalloidin, cell nuclei stained blue by DAPI. Glass – cells seeded on glass coverslips and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _plate – cells seeded on scaffolds in the well plates and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _v_static – cells seeded on scaffolds, enclosed in the vials (37 °C), Sc _v_RPM – cells seeded on scaffolds, enclosed in the vials (37 °C), and subjected to the 60 rpm (Experiment 1) and 10 rpm (Experiment 2) settings on the RPM. Scale bar =100 µm.

Journal: Biomaterials and Biosystems

Article Title: The influence of simulated microgravity on MG-63 osteoblast-like cells cultured on polymeric scaffold

doi: 10.1016/j.bbiosy.2026.100137

Figure Lengend Snippet: Live/dead staining results in Experiment 1 (A, C, E, G) and Experiment 2 (B, D, F, H) of MG-63 cells cultured on (A, B) Glass, (C, D) Sc _plate, (E, F) Sc _v_static, (G, H) Sc _v_RPM. Living cells stained green by calcein-AM, dead cells stained red by propidium iodide. Phalloidin/DAPI staining results in Experiment 2: (B2) Glass, (D2) Sc _plate, (F2) Sc _v_static, (H2) Sc _v_RPM. Actin filaments stained green by phalloidin, cell nuclei stained blue by DAPI. Glass – cells seeded on glass coverslips and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _plate – cells seeded on scaffolds in the well plates and cultured in standard conditions (37 °C, 5 % CO 2 ), Sc _v_static – cells seeded on scaffolds, enclosed in the vials (37 °C), Sc _v_RPM – cells seeded on scaffolds, enclosed in the vials (37 °C), and subjected to the 60 rpm (Experiment 1) and 10 rpm (Experiment 2) settings on the RPM. Scale bar =100 µm.

Article Snippet: Cell culture evaluation was performed using AlamarBlue Cell Viability test (ThermoFisher), lactate dehydrogenase activity assay (LDH, Takara, Saint-Germain-en-Laye, France), ALP activity assay based on p-nitrophenol (Sigma Aldrich), calcein-AM and propidium iodide (PI) staining (Sigma Aldrich), Alexa Fluor 488 phalloidin and 4′,6-diamidino-2-phenylindole, dihydrochloride (DAPI) staining (ThermoFisher).

Techniques: Staining, Cell Culture